Improved electrophoretic separation of polymorphic short tandem repeats in agarose gels using bis-benzimide.
نویسندگان
چکیده
منابع مشابه
Agarose-based system for separation of short tandem repeat loci.
Short tandem repeats (STRs) are traditionally analyzed on large polyacrylamide electrophoresis gels. We demonstrate in this study that a small (10-cm-long, 1-mm-thick) agarose gel is sufficient for analysis of multiplexed samples for several commonly used STR loci. A system was developed using a high-resolution agarose, MetaPhor. Within an hour of electrophoresis, sufficient resolution was obta...
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Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing. To reduce the drawback of preparing gels or using expensive precast gels, we have developed a simple and rapid method to reuse gels between 2 and 8 times over a period of several days. Following the initial elec...
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1Institute for Biochemistry and Protein Research, Zlnstitute for Molecular Genetics, Agricultural Biotechnology Center, H-2101, GOdOll6, Hungary Microsatellites are tandemly repeated short (1-6 bp) simple sequences that are highly abundant in most eukaryotic genomes. (1-4) Because of the variation in the number of repeats, these microsatellites show extensive length polymorphism. Thus, they are...
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عنوان ژورنال:
- BioTechniques
دوره 23 4 شماره
صفحات -
تاریخ انتشار 1997